Cell Signaling Technology

Product Pathways - Protein Folding/Stability

Ubc9 Antibody #4918

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 17 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

Ubc9 Antibody detects endogenous levels of total Ubc9 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding V148 of human Ubc9. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Ubc9 Antibody.

Background

The process of SUMO-1 conjugation is similar to that seen with ubiquitin and other forms of post-translational protein modification (1). Like ubiquitin, SUMO-1 is conjugated to its target protein by the coordinated action of ubiquitin conjugation enzymes E1, E2 and E3 (2). Ubc9 (or ube2M) is a highly conserved, 158 amino acid protein that acts as a SUMO-1 conjugating enzyme (3). Ubc9 binds to target proteins through their SUMO-1-CS (consensus sequence) domains and interacts with SUMO via the structurally conserved N-terminal domain (3,4). Localization of Ubc9 to the nucleus and the nuclear envelope allows this enzyme to catalyze target protein sumoylation and regulate target protein nucleocytoplasmic transport and transcriptional activity (5,6). Ubc9 target proteins include a host of proteins (RAD51, RAD52, p53 and c-Jun) that regulate the cell cycle, DNA repair, and p53-dependent processes (7).

  1. Geiss-Friedlander, R. and Melchior, F. (2007) Nat Rev Mol Cell Biol 8, 947-56.
  2. Tatham, M.H. et al. (2003) Biochemistry 42, 3168-79.
  3. Sampson, D.A. et al. (2001) J Biol Chem 276, 21664-9.
  4. Liu, Q. et al. (1999) J Biol Chem 274, 16979-87.
  5. Lee, G.W. et al. (1998) J Biol Chem 273, 6503-7.
  6. Pichler, A. and Melchior, F. (2002) Traffic 3, 381-7.
  7. Mo, Y.Y. and Moschos, S.J. (2005) Expert Opin Ther Targets 9, 1203-16.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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