Cell Signaling Technology

Product Pathways - NF-kB Signaling

IRF-7 Antibody #4920

Applications Reactivity Sensitivity MW (kDa) Source
W IP H (Mk) Endogenous 65 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

IRF-7 Antibody detects endogenous levels of IRF-7 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro115 of human IRF-7. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells treated with IFN-α (100 nM) for the indicated times, using IRF-7 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from DLD-1 cells, untreated or treated overnight with IFN-α (100 nM), using IRF-7 Antibody.

Background

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).

IRF-7, which is functionally similar to IRF-3, is preferentially expressed in lymphoid cells and induced by virus, LPS, and IFN-α (3-5). IRF-7 plays an essential role in the induction of type I interferon in response viral infecton (6-8). Like IRF-3, IRF-7 is regulated at multiple serine phosphorylation sites near its carboxyl terminus, which are required for nuclear translocation, DNA binding, and transcriptional activity (9-11).

  1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
  2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
  3. Au, W.C. et al. (1998) J Biol Chem 273, 29210-7.
  4. Wathelet, M.G. et al. (1998) Mol Cell 1, 507-18.
  5. Marié, I. et al. (1998) EMBO J 17, 6660-9.
  6. Sato, M. et al. (2000) Immunity 13, 539-48.
  7. Honda, K. et al. (2005) Nature 434, 772-7.
  8. Colina, R. et al. (2008) Nature 452, 323-8.
  9. Lin, R. et al. (2000) J Biol Chem 275, 34320-7.
  10. Yang, H. et al. (2003) J Biol Chem 278, 15495-504.
  11. Caillaud, A. et al. (2005) J Biol Chem 280, 17671-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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