Cell Signaling Technology

Product Pathways - NF-kB Signaling

RIP Antibody #4926

Applications Reactivity Sensitivity MW (kDa) Source
W H Mk Endogenous 78 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RIP Antibody detects endogenous levels of RIP (RIP1) protein. No cross-reactivity was detected with other family members. This antibody also detects a carboxy-terminal fragment of RIP (45 kDa) produced by caspase-8 dependent cleavage.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding arginine 413 of human RIP. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HL-60, MOLT-4 and Raji cell lines, using RIP Antibody.

Background

The RIP (receptor-interacting protein) family of serine-threonine kinases (RIP, RIP2, RIP3 and RIP4) are important regulators of cellular stress that can trigger pro-survival and inflammatory responses through the activation of NF-κB as well as pro-apoptotic pathways (1). In addition to the kinase domain, RIP contains a death domain responsible for interaction with the death domain receptor Fas and for the recruitment to TNFR1 through interaction with TRADD (2,3). RIP also interacts with TNF-receptor-associated factors (TRAFs) and can recruit IKKs to the TNFR1 signaling complex via interaction with NEMO leading to IκB phosphorylation and degradation (6,7). Overexpression of RIP induces both NF-κB activation and apoptosis (2,3). Caspase-8 dependent cleavage of the death domain on RIP can trigger the apoptotic activity of RIP (8). RIP-deficient cells show a failure in TNF-mediated NF-κB activation making the cells more sensitive to apoptosis (4,5).

  1. Meylan, E. and Tschopp, J. (2005) Trends Biochem Sci 30, 151-9.
  2. Hsu, H. et al. (1996) Immunity 4, 387-96.
  3. Stanger, B.Z. et al. (1995) Cell 81, 513-23.
  4. Ting, A.T. et al. (1996) EMBO J 15, 6189-96.
  5. Kelliher, M.A. et al. (1998) Immunity 8, 297-303.
  6. Devin, A. et al. (2000) Immunity 12, 419-29.
  7. Zhang, S.Q. et al. (2000) Immunity 12, 301-11.
  8. Lin, Y. et al. (1999) Genes Dev 13, 2514-26.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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