Cell Signaling Technology

Product Pathways - Apoptosis

Caspase-8 Antibody (Mouse Specific) #4927

Applications Reactivity Sensitivity MW (kDa) Source
W M Endogenous 45, 57 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Caspase-8 Antibody (Mouse Specific) detects endogenous levels of mouse caspase-8 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region within the large 18 kDa subunit of mouse caspase-8. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from BaF3, Raw264.7 and C2C12 cell lines, using Caspase-8 Antibody (Mouse Specific).

Background

Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.

  1. Muzio, M. et al. (1996) Cell 85, 817-827.
  2. Boldin, M.P. et al. (1996) Cell 85, 803-815.
  3. Fernandes-Alnemri, T. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 7464-7469.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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