Product Pathways - Apoptosis
Granzyme A Antibody #4928
PhosphoSitePlus® protein, site, and accession data: GZMA
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W E-P | H | Endogenous | 28 | Rabbit |
Applications Key:
W=Western Blotting
E-P=ELISA (Peptide)
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4928:
- ELISA Peptide, Western Blotting
Specificity / Sensitivity
Granzyme A Antibody detects endogenous levels of Granzyme A protein. No cross-reactivity was observed with Granzyme B.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding threonine 121 of human Granzyme A. Antibodies were purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of recombinant His-tagged Granzyme A (10 ng) and lysates from the natural killer cell line NK-92, using Granzyme A Antibody.
ELISA-Peptide
ELISA analysis of plates coated with recombinant human Granzyme A, using Granzyme A Antibody.
Background
Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3).
- Trapani, J.A. (2001) Genome Biol. 2, REVIEWS 3014.
- Lord, S.J. et al. (2003) Immunol. Rev. 193, 31-8.
- Trapani, J.A. and Sutton, V.R. (2003) Curr. Opin. Immunol. 15, 533-43.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
