Cell Signaling Technology

Product Pathways - Tag Antibodies

Albumin Antibody #4929

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 67 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Albumin Antibody recognizes total human albumin protein. It does not recognize albumin from mouse, bovine or horse.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro134 of human albumin. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of human and mouse serum as well as cell lysates from HepG2 and HeLa cells, using Albumin Antibody. Equivalent of 0.1 nL of serum was analyzed in human and mouse samples.

Background

Serum albumin is the most abundant protein in plasma. It accounts for over 50% of total human plasma protein content, having a concentration of approximately 40 g/L. Albumin is predominantly synthesized in the liver and is a major transportation component for many endogenous and exogenous compounds, including fatty acids, steroid hormones, metabolites and drugs. It is also responsible for maintaining colloid osmotic pressure and may affect microvascular integrity (1).

  1. Quinlan, G.J. et al. (2005) Hepatology 41, 1211-1219.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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