Cell Signaling Technology

Product Pathways - Protein Stability

SUMO-1 Antibody #4930

Applications Reactivity Sensitivity Source
W IP IHC-P IF-IC H M R Mk Endogenous Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SUMO-1 Antibody detects recombinant SUMO-1 and endogenous levels of sumoylated proteins (e.g. SUMO-1-RanGAP, 90kD).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence within human SUMO-1 that does not correspond to SUMO-2/3. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of recombinant GST-SUMO-1 protein (38 kDa) and extracts from CAD, HeLa and PC-12 cells, using SUMO-1 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using SUMO-1 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SUMO-1 Antibody.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, usinig SUMO-1 Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, using SUMO-1 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 (red).

Background

Small ubiquitin-related modifier 1, 2 and 3 (SUMO-1, -2 and -3) are members of the ubiquitin-like protein family (1). The covalent attachment of the SUMO-1, -2 or -3 (SUMOylation) to target proteins is analogous to ubiquitination. This post-translational modification is a reversible, multi-step process that is initiated by cleaving a precursor protein to a mature protein. Mature SUMO-1, -2 or -3 is then linked to the activating enzyme E1, conjugated to E2 and in conjunction with E3, SUMO-1, -2 or -3 is ligated to the target protein (2). Ubiquitin and the individual SUMO family members are all targeted to different proteins with diverse biological functions. Ubiquitin predominantly regulates degradation of its target (1). In contrast, SUMO-1 is conjugated to RanGAP, PML, p53 and IκB-α to regulate nuclear trafficking, formation of subnuclear structures, regulation of transcriptional activity and protein stability (3-7). SUMO-2/-3 forms poly-(SUMO) chains, is conjugated to topoisomerase II and APP, regulates chromosomal segregation and cellular responses to environmental stress, and plays a role in the progression of Alzheimer disease (8-11).

  1. Schwartz, D.C. and Hochstrasser, M. (2003) Trends Biochem. Sci. 28, 321-328.
  2. Kim, K.I. et al. (2002) J. Cell Physiol. 191, 257-268.
  3. Matunis, M.J. et al. (1996) J. Cell Biol. 135, 1457-1470.
  4. Duprez, E. et al. (1999) J. Cell Sci. 112, 381-393.
  5. Gostissa, M. et al. (1999) EMBO J. 18, 6462-6474.
  6. Rodriguez, M.S. et al. (1999) EMBO J. 18, 6455-6461.
  7. Desterro, J.M. et al. (1998) Mol. Cell 2, 233-239.
  8. Tatham, M.H. et al. (2001) J. Biol. Chem. 276, 35368-35374.
  9. Azuma, Y. et al. (2003) J. Cell Biol. 163, 477-487.
  10. Li, Y. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 259-264.
  11. Saitoh, H. and Hinchey, J. (2000) J. Biol. Chem. 275, 6252-6258.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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