Product Pathways - Adhesion
Claudin-1 Antibody #4933
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H (M) (R) | Endogenous | 20 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Claudin-1 Antibody detects endogenous levels of total claudin-1 protein. Based on sequence similarity, the antibody may cross-react with claudin-2.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of mouse claudin-1. Antibodies are purified using protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from A431 and ZR-75 cells using Claudin-1 Antibody.
IP
Immunoprecipitation of claudin-1 from A431 cell extract using Claudin-1 Antibody. Western blot analysis was performed using the same antibody.
Background
Tight junctions, or zonula occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces. Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-1 is expressed primarily in keratinocytes (4) and normal mammary epithelial cells, but is absent or reduced in breast carcinomas and breast cancer cell lines (5,6).
- Shin, K. et al. (2006) Annu. Rev. Cell Dev. Biol. 22, 207-235.
- Oliveira, S.S. and Morgado-Diaz, J.A. (2007) Cell Mol. Life Sci. 64, 17-28.
- Hewitt, K.J. et al. (2006) BMC Cancer 6, 186.
- Brandner, J.M. et al. (2002) Eur. J. Cell Biol. 81, 253-263.
- Kramer, F. et al. (2000) Hum. Genet. 107, 249-256.
- Swisshelm, K. et al. (1999) Gene 226, 285-295.
Application References
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Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
