Cell Signaling Technology

Product Pathways - Apoptosis

Acinus Antibody #4934

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC H M R Mk Endogenous 220, 84, 86 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Acinus Antibody detects endogenous levels of total Acinus protein. The antibody recognizes L, S and S' isoforms.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala835 of Acinus. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using Acinus Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Acinus Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red).

Background

Acinus (apoptotic chromatin condensation inducer in the nucleus) is a caspase substrate that has been implicated in nuclear changes during apoptosis (1). Chromatin condensation and DNA fragmentation are both nuclear morphological features associated with apoptosis. Acinus is expressed in different isoforms (L, S, S') most likely generated by alternative splicing (1). During apoptosis Acinus is cleaved by caspase-3 to generate a 23 kDa fragment that was reported to induce chromatin condensation (1). Acinus has been identified to be a component of the spliceosome complex, ASAP, suggesting a role in pre-mRNA processing (2-4). Down regulation of Acinus by RNA interference inhibits cell growth (5). This study also found that loss of Acinus inhibits DNA fragmentation but not chromatin condensation during apoptosis.

  1. Sahara, S. et al. (1999) Nature 401, 168-173.
  2. Schwerk, C. et al. (2003) Mol. Cell. Biol. 23, 2981-2990.
  3. Zhou, Z. et al. (2002) Nature 419, 182-185.
  4. Rappsilber, J. et al. (2002) Genome Res. 12, 1231-1245.
  5. Joselin, A.P. et al. (2006) J. Biol. Chem. 281, 12475-12484.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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