Cell Signaling Technology

Product Pathways - Translational Control

Rheb Antibody #4935

Applications Reactivity Sensitivity MW (kDa) Source
W IHC-P F H M R Sc Dm (B) Endogenous 21 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Sc=S. cerevisiae  B=Bovine  Dm=D. melanogaster
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

Rheb Antibody detects endogenous levels of Rheb. The antibody does not cross-react with other family members at physiological levels.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn50 of Rheb. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Raji, Ramos, and NBT-II cells, using Rheb Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brain, showing cytoplasmic localization, using Rheb Antibody in the presence of control peptide (left) or antigen specific peptide (right).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells, using Rheb antibody (blue) compared to a nonspecific negative control antibody (red).


Background

Rheb (Ras Homolog Enriched in Brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure (1). While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Like other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway (2). Biochemical and genetic studies demonstrate that Rheb has an important role in regulating the insulin/TOR signaling pathway (3-6). The mammalian target of rapamycin, mTOR, is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth. The tuberin/hamartin (TSC2/TSC1) complex inhibits mTOR activity indirectly by inhibiting Rheb via tuberin's GAP activity (7).

  1. Yamagata, K. et al. (1994) J. Biol. Chem. 269, 16333-16339.
  2. Yee, W.M. and Worley, P.F. (1997) Mol. Cell. Biol. 17, 921-933.
  3. Inoki, . et al. (2003) Genes Dev. 17, 1829-1834.
  4. Stocker, H. et al. (2003) Nature Cell Biol. 5, 559-565.
  5. Saucedo, L. J. et al. (2003) Nat. Cell Biol. 5, 566-571.
  6. Zhang, Y. et al. (2003) Nat. Cell Biol. 5, 578-581.
  7. Li, Y. et al. (2004) Trends Biochem. Sci. 1, 32-38.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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