Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Axl (C2B12) Rabbit mAb #4939

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Mk Pg Endogenous 138 kDa Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Axl (C2B12) Rabbit mAb detects endogenous levels of Axl protein. The antibody does not cross-react with related proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human Axl proteins.

Western Blotting

Western Blotting

Western blot analysis of cell extracts from various cell lines, using Axl (C2B12) Rabbit mAb.

Background

Axl, Sky, and Mer are three members of a receptor tyrosine kinase (RTK) family that share a conserved intracellular tyrosine kinase domain and an extracellular domain similar to those seen in cell adhesion molecules. These RTKs bind the vitamin K-dependent protein growth-arrest-specific 6 (Gas6), which is structurally related to the protein S anticoagulation factor (1). Upon binding to its receptor, Gas6 activates phosphatidylinositol 3-kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-κB (2,3). A large body of evidence supports a role for Gas6/Axl signaling in cell growth and survival in normal and cancer cells (4).

  1. Crosier, K.E. and Crosier, P.S. (1997) Pathology 29, 131-135.
  2. Demarchi, F. et al. (2001) J. Biol. Chem. 276, 31738-31744.
  3. Lee, W. P. et al. (2002) Oncogene 21, 329-336.
  4. Bellosta, P. et al. (1997) Oncogene 15, 2387-2397.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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