Product Pathways - Tyrosine Kinase/ Adaptors
Axl (C2B12) Rabbit mAb #4939
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H Mk Pg | Endogenous | 138 kDa | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Mk=Monkey
Pg=Pig
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Axl (C2B12) Rabbit mAb detects endogenous levels of Axl protein. The antibody does not cross-react with related proteins.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with recombinant human Axl proteins.
Western Blotting
Western blot analysis of cell extracts from various cell lines, using Axl (C2B12) Rabbit mAb.
Background
Axl, Sky and Mer are three members of a recently characterized receptor tyrosine kinase (RTK) family that share a conserved intracellular tyrosine kinase domain and an extracellular domain similar to those seen in cell adhesion molecules. These RTKs bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6), which is structurally related to the protein S anticoagulation factor (1). Upon binding to its receptor, Gas6 activates phosphatidylinositol 3-kinase (PI3K) and its downstream targets S6K and Akt, as well as NF-κB (2,3). A large body of evidence supports a role for Gas6/Axl signaling in cell growth and survival in normal and cancer cells (4).
- Crosier, K.E. and Crosier, P.S. (1997) Pathology 29, 131-135.
- Demarchi, F. et al. (2001) J. Biol. Chem. 276, 31738-31744.
- Lee, W. P. et al. (2002) Oncogene 21, 329-336.
- Bellosta, P. et al. (1997) Oncogene 15, 2387-2397.
Application References
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Companion Products
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
