Product Pathways - NF-kappaB Signaling
IRF-2 Antibody #4943
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R (Mk) | Endogenous | 45 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
IRF-2 Antibody detects endogenous levels of total IRF-2 protein.
Source / Purification
Polyclonal antibodies were prepared by immunizing rabbits with a synthetic peptide (KLH-coulpled) corresponding to residues surrounding Asp327 of human IRF-2. Antibodies were purified by affinity chromatography.
Western Blotting
Western blot analysis of extracts from SR, Ramos and A20 cell lines using IRF-2 Antibody.
Background
Interferon regulatory factors (IRFs) comprise a family of transcription factors that function with the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in the pathogen defense, autoimmunity, lymphocyte development, cell growth and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7 and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS) and IFN regulatory elements (IRF-E) (2).
Structurally similar to other IRF family members, IRF-2 acts as both a transcription activator and repressor. IRF-2 promotes transcription of several genes, including histone H4, the VCAM1 cell adhesion protein, and CIITA, a MHC transactivator protein. Conversely, IRF-2 competively binds to promoter sites utilized by IRF-1, repressing activation by this related transcription factor (3). Its activity may be controlled by inducible proteolysis (4). IRF-2 has also been associated with increased oncogenic potential (5,6) and lymphocyte development (7,8).
- Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
- Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
- Harada, H. et al. (1989) Cell 58, 729-739.
- Palombella, V.J. and Maniatis, T. (1992) Mol. Cell Biol. 12, 3325-3336.
- Harada, H. et al. (1993) Science 259, 971-974.
- Nguyen, H. et al. (1995) Oncogene 11, 537-544.
- Matsuyama, T. et al. (1993) Cell 75, 83-97.
- Lohoff, M. et al. (2000) J. Exp. Med. 192, 325-336.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
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- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
