Product Pathways - DNA Damage
Phospho-FANCD2 (Ser222) Antibody #4945
PhosphoSitePlus® protein, site, and accession data: FANCD2
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H Mk | Endogenous | 155 FANCD2-S. 230 FANCD2-L. | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4945:
- Western Blotting
Specificity / Sensitivity
Phospho-FANCD2 (Ser222) Antibody detects endogenous levels of FANCD2 only when phosphorylated at Ser222.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser222 of human FANCD2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from untreated or UV-treated (50 mJ/cm2, 1hr) ME-180 and HT-29 cells, using Phospho-FANCD2 (Ser222) Antibody.
Background
Fanconi anemia (FA) is an autosomal recessive genetic disorder resulting in symptoms that include chromosomal breakage, bone marrow failure, hypersensitivity to DNA cross-linking agents (such as MMC), and a predisposition to cancer (1). FANCD2 is the gene product of one of at least six genes thought to contribute heterogeneously to FA (2). MMC treatment results in ubiquitination of FANCD2 on Lys561, converting the protein from a short form (FANCD2-S) to a monoubiquitinated or long (FANCD2-L) form that localizes to subnuclear foci at sites of DNA damage with BRCA1, Rad51, and NBS1 (3-5). Ionizing radiation, on the other hand, results in ATM-dependent phosphorylation of FANCD2 at Ser222 and Ser1404 resulting in an S-phase arrest (4).
- Alter, B.P. (1996) Am. J. Hematol. 53, 99-110.
- Timmers, C. et al. (2001) Mol. Cell 7, 241-248.
- Garcia-Higuera, I. et al. (2001) Mol. Cell 7, 249-262.
- Taniguchi, T. et al. (2002) Cell 109, 459-472.
- Nakanishi, K. et al. (2002) Nat. Cell Biol. 4, 913-920.
Application References
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
