Cell Signaling Technology

Product Pathways - DNA Damage

Phospho-FANCD2 (Ser222) Antibody #4945

Applications Reactivity MW (kDa) Source
W H Mk 155 FANCD2-S. 230 FANCD2-L. Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-FANCD2 (Ser222) Antibody detects endogenous levels of FANCD2 only when phosphorylated at Ser222.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser222 of human FANCD2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from untreated or UV-treated (50 mJ/cm2, 1hr) ME-180 and HT-29 cells, using Phospho-FANCD2 (Ser222) Antibody.

Background

Fanconi anemia (FA) is an autosomal recessive genetic disorder resulting in symptoms that include chromosomal breakage, bone marrow failure, hypersensitivity to DNA cross-linking agents (such as MMC), and a predisposition to cancer (1). FANCD2 is the gene product of one of at least six genes thought to contribute heterogeneously to FA (2). MMC treatment results in ubiquitination of FANCD2 on Lys561, converting the protein from a short form (FANCD2-S) to a monoubiquitinated or long (FANCD2-L) form that localizes to subnuclear foci at sites of DNA damage with BRCA1, Rad51, and NBS1 (3-5). Ionizing radiation, on the other hand, results in ATM-dependent phosphorylation of FANCD2 at Ser222 and Ser1404 resulting in an S-phase arrest (4).

  1. Alter, B.P. (1996) Am. J. Hematol. 53, 99-110.
  2. Timmers, C. et al. (2001) Mol. Cell 7, 241-248.
  3. Garcia-Higuera, I. et al. (2001) Mol. Cell 7, 249-262.
  4. Taniguchi, T. et al. (2002) Cell 109, 459-472.
  5. Nakanishi, K. et al. (2002) Nat. Cell Biol. 4, 913-920.

Application References

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