Product Pathways - NF-kB Signaling
IRF-4 (P173) Antibody #4948
|W||H M R (Mk)||Endogenous||50||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
IRF-4 (P173) Antibody detects endogenous levels of total IRF-4 protein. This antibody does not cross-react with other family members at physiological levels.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around Pro173 of human IRF-4 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from KARPAS-620 (human) and A20 (mouse) cells using IRF-4 Antibody #4964 and IRF-4 (P173) Antibody #4948.
Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).
IRF-4 was independently cloned by three groups and demonstrated to have roles in different contexts of lymphoid regulation (3-5). First, IRF-4 (Pip) was found to associate with PU.1, a member of the ETS family specific to hematopoietic cells, and to regulate the expression of B cell-specific genes (3). Second, it was characterized as a lymphoid-specific member of the IRF family (LSIRF) and able to bind to ISRE (4). Third, it was identified in activated T cells as a factor that binds to the promoter of the interleukin-5 gene (ICSAT), and shown to repress gene activation induced by IFN (5). IRF-4 is expressed in all stages of B cell development and in mature T cells, and is inducible in primary lymphocytes by antigen mimetic stimuli such as Concavalin A, CD3 crosslinking, anti-IgM and PMA treatment (4,5). Mice deficient in IRF-4 show normal distribution of B and T lymphocytes at 4 to 5 weeks, but later develop progressive generalized lymphadenopathy, suggesting a role for IRF-4 in the function and homeostasis of mature B and T lymphocytes (6).
- Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
- Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
- Eisenbeis, C.F. et al. (1995) Genes Dev. 9, 1377-1387.
- Matsuyama, T. et al. (1995) Nucleic Acids Res. 23, 2127-2136.
- Yamagata, T. et al. (1996) Mol. Cell Biol. 16, 1283-1294.
- Mittrücker, H.W. et al. (1997) Science 275, 540-543.
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For Research Use Only. Not For Use In Diagnostic Procedures.