Cell Signaling Technology

Product Pathways - NF-kB Signaling

IRF-5 Antibody (Rodent Specific) #4950

Applications Reactivity Sensitivity MW (kDa) Source
W IP M R Endogenous 60 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

IRF-5 Antibody (Rodent Specific) detects endogenous levels of total IRF-5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser172 of mouse IRF-5 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Raw264.7 cells, untreated or LPS-treated (1 µg/ml for 6 h), M12.4.1 cells and A20 cells, using IRF-5 Antibody (Rodent Specific).

Western Blotting

Western Blotting

Western blot analysis of extracts from A20 and BaF3 cells using IRF-5 Antibody (Rodent Specific).

Background

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).

IRF-5 is expressed in lymphoid tissues and peripheral blood lymphocytes and participates in the induction of type I interferon genes following viral infection (3). Activation of IRF-5 signaling is triggered by components of the toll-like receptor (TLR) pathway, including TLR7 and MyD88 (4,5). Genetic variants of IRF-5 have been associated with disorders where the IFN pathway is abnormally activated, such as systemic lupus erythematosus (6,7).

  1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
  2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
  3. Barnes, B.J. et al. (2001) J. Biol. Chem. 276, 23382-23390.
  4. Takaoka, A. et al. (2005) Nature 434, 243-249.
  5. Schoenemeyer, A. et al. (2005) J. Biol. Chem. 280, 17005-17012.
  6. Sigurdsson, S. et al. (2005) Am. J. Hum. Genet. 76, 528-537.
  7. Graham, G. et al. (2006) Nat. Genet. 38, 550-555.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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