Cell Signaling Technology

Product Pathways - Apoptosis / Autophagy

c-IAP1 Antibody #4952

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 62 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

c-IAP1 Antibody detects endogenous levels of c-IAP1. At physiological levels, cross reactivity was not detected with other family members.

Source / Purification

Polyclonal antibodies are pruduced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region between the first two BIR domains of c-IAP1. Antibodies are purified by protein A and affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat, HeLa, and HT29 cells, using c-IAP1 Antibody.

Background

The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of the family include c-IAP1, c-IAP2, XIAP, Surivivin, Livin and NAIP. Overexpression of IAP family members, particularly Survivin and Livin, in cancer cell lines and primary tumors suggest an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7 and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks its interaction with caspase-9, thereby allowing the processing and activation of the caspase (7).

  1. Deveraux, Q.L. and Reed, J.C. (1999) Genes Dev 13, 239-52.
  2. Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23.
  3. Altieri, D.C. et al. (1999) Lab Invest 79, 1327-33.
  4. Tamm, I. et al. (2000) Clin Cancer Res 6, 1796-803.
  5. Kasof, G.M. and Gomes, B.C. (2001) J Biol Chem 276, 3238-46.
  6. Deveraux, Q.L. et al. (1997) Nature 388, 300-4.
  7. Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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