Product Pathways - Phosphatases

PP2A A Subunit (4G7) Mouse mAb #4955

Applications	Reactivity	MW (kDa)	Source	Isotype
W	H M R Mk	62	Mouse	IgG1

Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

PP2A A Subunit (4G7) Mouse mAb detects endogenous levels of total PP2A A subunit, both alpha and beta isoforms. This antibody does not cross-react with other PP2A subunits.

Source / Purification

Monoclonal antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of human PP2A A subunit.

Western Blotting

Western blot analysis of extracts from PL45, L929, C6 and COS cells, using PP2A A Subunit (4G7) Mouse mAb.

Background

Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by α and β isoforms (1). Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regulatory protein families contain α, β, γ and δ isoforms, with the B' family also including an ε protein. B'' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B''' regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit (1). This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity (4). Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed (5,6). Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits (6-8).

Application References

Ogris, E. et al. (1999) A protein phosphatase methylesterase (PME-1) is one of several novel proteins stably associating with two inactive mutants of protein phosphatase 2A. J Biol Chem. 1999 May 14;274(20):14382-91. . J. Biol. Chem. 274, 14382-14391. This article references the use of PP2A A Subunit (4G7) Mouse mAb in the following applications: Western Blotting
Yu, X. X. et al. (2001) Methylation of the protein phosphatase 2A catalytic subunit is essential for association of Balpha regulatory subunit but not SG2NA, striatin, or polyomavirus middle tumor antigen. Mol. Biol. Cell 12, 185-199. This article references the use of PP2A A Subunit (4G7) Mouse mAb in the following applications: Western Blotting
Moreno, C. S. et al. (2000) WD40 repeat proteins striatin and S/G(2) nuclear autoantigen are members of a novel family of calmodulin-binding proteins that associate with protein phosphatase 2A. J. Biol. Chem. 275, 5257-5263. This article references the use of PP2A A Subunit (4G7) Mouse mAb in the following applications: Western Blotting

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