Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

SLP-76 Antibody #4958

Applications Reactivity Sensitivity MW (kDa) Source
W IP IHC-P H M Endogenous 76 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SLP-76 Antibody detects endogenous levels of total SLP-76 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human SLP-76. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and BW5147cells using SLP-76 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon using SLP-76 Antibody in the presence of control peptide (left) or antigen-specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using SLP-76 Antibody.


Background

SH2 domain-containing leukocyte protein of 76 kDa (SLP-76) is a hematopoietic adapter protein that is important in multiple biochemical signaling pathways and necessary for T cell development and activation (1). ZAP-70 phosphorylates SLP-76 and LAT as a result of TCR ligation. SLP-76 has amino-terminal tyrosine residues followed by a proline rich domain and a carboxy-terminal SH2 domain. Phosphorylation of Tyr113 and Tyr128 result in recruitment of the GEF Vav and the adapter protein Nck (2). TCR ligation also mediates an association between SLP-76 and Itk, which is accomplished in part via the proline rich domain of SLP-76 and the SH3 domain of ITK. Furthermore, the proline rich domain of SLP-76 binds to the SH3 domains of Grb2-like adapter Gads (3-4). In resting cells, SLP-76 is predominantly in the cytosol. Upon TCR ligation, SLP-76 translocates to the plasma membrane and promotes the assembly of a multi-protein signaling complex that includes Vav, Nck, Itk and PLCg1 (1). The expression of SLP-76 is tightly regulated: the protein is detected at very early stages of thymocyte development, increases as thymocyte maturation progresses, and is reduced as cells mature to CD4+ CD8+ double-positive thymocytes (5).

  1. Clements, J.L. (2003) Immunol. Rev. 191, 211-219.
  2. Bubeck Wardenburg, J. et al. (1998) Immunity. 9, 607-616.
  3. Bunnell, S. C. et al. (2000) J. Biol. Chem. 275, 2219-2230.
  4. Liu, S. K. et al. (1999) Curr. Biol. 9, 67-75.
  5. Clements, J. L. et al. (1998) J. Immunol. 161, 3880-3889.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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