Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

AID (EK2 5G9) Rat mAb #4959

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P H Endogenous 24 Rat IgG2b

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

AID (EK2 5G9) Rat mAb detects endogenous levels of total AID protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Asp191 of human AID.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from P3HR-1 and Ramos cells using AID (EK2 5G9) Rat mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using AID (EK2 5G9) Rat mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using AID (EK2 5G9) Rat mAb. (Image courtesy of Paula Smith and Dr. Riccardo Dalla-Favera, Columbia Univerisity, New York, NY.)


Background

Activation-induced cytidine deaminase (AID) is thought to modify RNA due to its high homology to the RNA editing enzyme APOBEC-1. This function, however, has not been confirmed in in vitro studies, which show that AID has significant cytidine deaminase activity, and that this activity is blocked by zinc chelation (1).The B cell immune system must specifically recognize several infectious agents, which vastly outnumber immunoglobulin gene segments present in a given organism. Mechanisms such as somatic hypermutation, isotype switch recombination and gene conversion introduce diversity and specificity to the immune system. Analysis of mouse models and patients with AID deficiency has established a link between all three of these mechanisms and AID function (2). AID protein is detected in germinal center centroblast and germinal center derived lymphomas (Burkitt lymphoma), but not in pre-germinal center B cells or post-germinal center neoplasms (B cell chronic lymphocytic leukemia and multiple myeloma) (3).

  1. Muramatsu, M. et al. (1999) J. Biol. Chem. 274, 18470-18476.
  2. Reynaud, C.A. et al. (2003) Nat. Immunol. 7, 631-638.
  3. Pasqualucci, L. et al. (2004) Blood 104, 3318-3325.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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