Product Pathways - Cytoskeletal Signaling

β-Actin Antibody #4967

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• application references
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Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IHC-P	H M R Mk Mi Hm B Dm Z (Pg) (C) (X) (Dg) (Hr)	Endogenous	45	Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Mi=Mink  Pg=Pig  C=Chicken  Hm=Hamster  B=Bovine  Dm=D. melanogaster  X=Xenopus  Z=Zebrafish  Dg=Dog  Hr=Horse
Species cross-reactivity is determined by Western blot.

Protocols

4967:

IHC / Paraffin, Western Blotting

Specificity / Sensitivity

β-Actin Antibody detects endogenous levels of β-actin. This antibody may cross-react with the γ-actin (cytoplasmic) isoform. It does not cross-react with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of human β-actin. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteosome dependent muscle proteolysis (6).

1. Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48-55.
2. Condeelis, J. (2001) Trends Cell Biol. 11, 288-293.
3. Lim, Y.P. et al. (2004) Clin. Cancer Res. 10, 3980-3987.
4. Kayalar, C. et al. (1996) Proc. Natl. Acad. Sci. USA. 93, 2234-2238.
5. Communal, C. et al. (2002) Proc. Natl. Acad. Sci. USA. 99, 6252-6256.
6. Du, J. et al. (2004) J. Clin. Invest. 113, 115-123.

Application References

• Bish, R.A. and Myers, M.P. (2007) J Biol Chem 282, 23184-93. Applications: Western Blotting
• Nociari, M. et al. (2007) J Virol 81, 4145-57. Applications: Western Blotting
• Wei, F. et al. (2007) J Biol Chem 282, 21551-60. Applications: Western Blotting
• Guo, A. et al. (2008) Proc Natl Acad Sci U S A 105, 692-7. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 3311 Phospho-Cofilin (Ser3) Antibody
• 3312 Cofilin Antibody
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 4968 Pan-Actin Antibody
• 4970 β-Actin (13E5) Rabbit mAb
• 1025 β-Actin Blocking Peptide

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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.