Product Pathways - Cytoskeletal Signaling
Pan-Actin Antibody #4968
|4968S||100 µl (10 western blots)||---||In Stock||---|
|4968||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat, Monkey, Zebrafish||Endogenous||45||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin)
Species predicted to react based on 100% sequence homology: D. melanogaster, Xenopus, Bovine, Pig.
Specificity / Sensitivity
Pan-Actin Antibody detects endogenous levels of total actin (all isoforms). The antibody also detects the 30 kDa actin fragment cleaved at glutamate 107.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp244 of human beta-actin. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from HeLa, L929, C6 and COS cells, using Pan-Actin Antibody.
Immunohistochemical analysis of paraffin-embedded human colon (smooth muscle), using Pan-Actin Antibody.
Immunohistochemical analysis of paraffin-embedded human heart, using Pan-Actin Antibody.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle, using Pan-Actin Antibody.
Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (6).
- Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48-55.
- Condeelis, J. (2001) Trends Cell Biol. 11, 288-293.
- Lim, Y.P. et al. (2004) Clin. Cancer Res. 10, 3980-3987.
- Kayalar, C. et al. (1996) Proc. Natl. Acad. Sci. USA. 93, 2234-2238.
- Communal, C. et al. (2002) Proc. Natl. Acad. Sci. USA. 99, 6252-6256.
- Du, J. et al. (2004) J. Clin. Invest. 113, 115-123.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 4967 β-Actin Antibody
- 3311 Phospho-Cofilin (Ser3) Antibody
- 3312 Cofilin Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 4970 β-Actin (13E5) Rabbit mAb
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 8112 SignalStain® Antibody Diluent
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
- 5425 Normal Goat Serum
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.