Cell Signaling Technology

Product Pathways - Apoptosis

Endonuclease G Antibody #4969

Applications Reactivity Sensitivity MW (kDa) Source
W H M R (Mk) Endogenous 25, 30 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Endonuclease G Antibody detects endogenous levels of total Endonuclease G protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys122 of human Endonuclease G. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat liver, using Endonuclease G Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells transfected with mouse EndoG, using Endonuclease G Antibody.

Background

Endonuclease G (EndoG) is a nuclear-encoded mitochondrial nuclease that has been reported to function in apoptosis, DNA recombination and cell proliferation (1-5). EndoG is expressed as a precursor protein containing an amino-terminal mitochondrial targeting sequence which is removed when the protein is imported into the mitochondria (1). During apoptosis EndoG is released from the mitochondria, translocates to the nucleus and cooperates with other nucleases to trigger DNA fragmentation associated with the apoptotic process (3,6,7).

  1. Côté, J. and Ruiz-Carrillo, A. (1993) Science 261, 765-769.
  2. Parrish, J. et al. (2001) Nature 412, 90-94.
  3. Li, L.Y. et al. (2001) Nature 412, 95-99.
  4. Zhang, J. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 15782-15787.
  5. Huang, K.J. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 8995-9000.
  6. Widlak, P. et al. (2001) J. Biol. Chem. 276, 48404-48409.
  7. Arnoult, D. et al. (2003) EMBO J. 22, 4385-4399.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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