Product Pathways - Cytoskeletal Signaling

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β-Actin (13E5) Rabbit mAb #4970

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IHC-P IHC-F IF-IC F	H M R Mk B Pg (Hm) (C) (Dg) (Hr)	Endogenous	45	Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  C=Chicken  B=Bovine  Dg=Dog  Pg=Pig  Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

4970:

Flow, IHC / Frozen, IHC / Paraffin, Immunofluorescence*, Western Blotting

* Product-specific protocol.

Specificity / Sensitivity

β-Actin (13E5) Rabbit mAb detects endogenous levels of total β-actin protein. This antibody may cross-react with the γ-actin (cytoplasmic isoform). It does not cross-react with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human β-actin protein.

Background

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (6).

1. Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48-55.
2. Condeelis, J. (2001) Trends Cell Biol. 11, 288-293.
3. Lim, Y.P. et al. (2004) Clin. Cancer Res. 10, 3980-3987.
4. Kayalar, C. et al. (1996) Proc. Natl. Acad. Sci. USA. 93, 2234-2238.
5. Communal, C. et al. (2002) Proc. Natl. Acad. Sci. USA. 99, 6252-6256.
6. Du, J. et al. (2004) J. Clin. Invest. 113, 115-123.

Application References

• Zhang, H. et al. (2009) J Biol Chem 284, 13355-62. Applications: Western Blotting
• Adon, A.M. et al. (2010) Mol Cell Biol 30, 694-710. Applications: Western Blotting
• Zhang, S. et al. (2009) Mol Cancer Res 7, 570-80. Applications: Western Blotting
• Wei, S.G. et al. (2009) Am J Physiol Heart Circ Physiol 296, H1425-33. Applications: Western Blotting
• Ikner, A. and Ashkenazi, A. (2011) J Biol Chem 286, 21546-54. Applications: Western Blotting
• Ippagunta, S.K. et al. (2011) Nat Immunol 12, 1010-6. Applications: Western Blotting
• Sadighi Akha, A.A. et al. (2011) J Biol Chem 286, 30344-51. Applications: Western Blotting
• Ikner, A. and Ashkenazi, A. (2011) J Biol Chem 286, 21546-54. Applications: Western Blotting
• Zheng, Y.S. et al. (2011) Oncogene , . Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 3311 Phospho-Cofilin (Ser3) Antibody
• 3312 Cofilin Antibody
• 4967 β-Actin Antibody
• 1025 β-Actin Blocking Peptide
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 4968 Pan-Actin Antibody
• 9967 Actin Reorganization Antibody Sampler Kit
• 8112 SignalStain^® Antibody Diluent
• 8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit)
• 5425 Normal Goat Serum
• 9997 Tris Buffered Saline with Tween 20 (TBST-10X)

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

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For Research Use Only. Not For Use In Diagnostic Procedures.