Product Pathways - Apoptosis / Autophagy
Puma Antibody #4976
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W IHC-P IF-IC F | H M R | 18, 23 | Rabbit |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Puma Antibody detects endogenous levels of Puma-alpha and Puma-beta. The antibody does not cross-react with other Bcl-2 family members at physiological levels.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy-terminal region of human Puma-alpha and -beta. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from K562, HT1080, PANC1, A204 and SR cells, using Puma Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, using Puma Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Puma Antibody in the presence of control peptide (left) or antigen-specific peptide (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Puma Antibody.
Flow Cytometry
Flow cytometric analysis of K562 cells, using Puma Antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of C2C12 cells labeled with Puma Antibody (left, blue) showing colocalization with mitochondria that have been labeled with MitoTracker® Red CMXRos (red, right). Actin filaments are shown labeled with fluorescein phalloidin (green).
Background
Puma (p53 upregulated modulator or apoptosis) is a -BH3-only+ Bcl-2 family member originally identified in differential gene expression studies as a p53-inducible gene (1,2). The "BH3-only" family members include Bad, Bid, Bik, Hrk, Bim and Noxa, all of which contain a BH3 domain but lack other conserved domains, BH1 and BH2, and generally promote apoptosis by binding to and antagonizing anti-apoptotic Bcl-2 family members through BH3 domain interactions (3). Two BH3-containing proteins are produced from the puma gene, Puma-alpha and Puma-beta, both of which are induced by p53, bind Bcl-2 and Bcl-xL, localize to the mitochondria and promote cyctochrome c release and apoptosis (1,2). Puma plays a critical role in the p53 tumor suppressor pathway. Targeted disruption of the puma gene impairs p53-mediated apoptosis and tumor suprression (4-7). Puma knockout mice show defects from multiple apoptotic stimuli, including ionizing irradiation, deregulated c-Myc expression and cytokine withdrawal (4).
- Yu, J. et al. (2001) Mol. Cell 7, 673-682.
- Nakano, K. and Vousden, K.H. (2001) Mol. Cell 7, 683-694.
- Bouillet, P. and Strasser, A. (2002) J. Cell Sci. 115, 1567-1574.
- Jeffers, J.R. et al. (2003) Cancer Cell 4, 321-328.
- Hemann, M.T. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 9333-9338.
- Yu, J. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 1931-1936.
- Villunger, A. et al. (2003) Science 302, 1036-1038.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
