Cell Signaling Technology

Product Pathways - Apoptosis

Puma Antibody #4976

Applications Reactivity Sensitivity MW (kDa) Source
W H (Mk) Endogenous 23 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Puma Antibody detects endogenous levels of total Puma protein. The antibody also cross-reacts with an 18kDa band of unknown origin.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal region of human Puma. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cells using Puma Antibody.

Background

Puma (p53 upregulated modulator of apoptosis) is a "BH3-only" Bcl-2 family member originally identified in differential gene expression studies as a p53-inducible gene (1,2). The "BH3-only" family members include Bad, Bid, Bik, Hrk, Bim, and Noxa, all of which contain a BH3 domain but lack other conserved domains, BH1 and BH2, and generally promote apoptosis by binding to and antagonizing anti-apoptotic Bcl-2 family members through BH3 domain interactions (3). Two BH3-containing proteins are produced from the puma gene, Puma-α and Puma-β, both of which are induced by p53, bind Bcl-2 and Bcl-xL, localize to the mitochondria, and promote cyctochrome c release and apoptosis (1,2). Puma plays a critical role in the p53 tumor suppressor pathway. Targeted disruption of the puma gene impairs p53-mediated apoptosis and tumor suprression (4-7). Puma knockout mice show defects from multiple apoptotic stimuli, including ionizing irradiation, deregulated c-Myc expression, and cytokine withdrawal (4).

  1. Yu, J. et al. (2001) Mol. Cell 7, 673-682.
  2. Nakano, K. and Vousden, K.H. (2001) Mol. Cell 7, 683-694.
  3. Bouillet, P. and Strasser, A. (2002) J. Cell Sci. 115, 1567-1574.
  4. Jeffers, J.R. et al. (2003) Cancer Cell 4, 321-328.
  5. Hemann, M.T. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 9333-9338.
  6. Yu, J. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 1931-1936.
  7. Villunger, A. et al. (2003) Science 302, 1036-1038.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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