Cell Signaling Technology

Product Pathways - Tyrosine Kinase/ Adaptors

Axl Antibody #4977

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Hm Endogenous 138 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Hm=Hamster
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Axl Antibody detects endogenous levels of Axl protein. The antibody does not cross-react with related proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to sequences surrounding amino acid residue 740 of human Axl. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using Axl Antibody.

Background

Axl, Sky and Mer are three members of a recently characterized receptor tyrosine kinase (RTK) family that share a conserved intracellular tyrosine kinase domain and an extracellular domain similar to those seen in cell adhesion molecules. These RTKs bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6), which is structurally related to the protein S anticoagulation factor (1). Upon binding to its receptor, Gas6 activates phosphatidylinositol 3-kinase (PI3K) and its downstream targets S6K and Akt, as well as NF-κB (2,3). A large body of evidence supports a role for Gas6/Axl signaling in cell growth and survival in normal and cancer cells (4).

  1. Crosier, K.E. and Crosier, P.S. (1997) Pathology 29, 131-135.
  2. Demarchi, F. et al. (2001) J. Biol. Chem. 276, 31738-31744.
  3. Lee, W. P. et al. (2002) Oncogene 21, 329-336.
  4. Bellosta, P. et al. (1997) Oncogene 15, 2387-2397.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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