Cell Signaling Technology

Product Pathways - Translational Control

Raptor Antibody #4978

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 150 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Raptor Antibody detects endogenous levels of total raptor protein. This antibody also cross-reacts with an unidentified protein of 90 kD.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues near the amino-terminus of human Raptor. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and 293 cells using Raptor Antibody.

Background

The regulatory associated protein of mTOR (Raptor) was identified as an mTOR binding partner that mediates mTOR signaling to downstream targets (1,2). Raptor binds to mTOR substrates, including 4E-BP1 and p70 S6 kinase, through their TOR signaling (TOS) motifs and is required for mTOR-mediated phosphorylation of these substrates (3,4). Binding of the FKBP12-rapamycin complex to mTOR inhibits the mTOR-raptor interaction, suggesting a mechanism for rapamycin's specific inhibition of mTOR signaling (5). This mTOR-raptor interaction and its regulation by nutrients and/or rapamycin is dependent on a protein called GβL (6). GβL is also part of the rapamycin-insensitive complex between mTOR and rictor (rapamycin-insensitive companion of mTOR), and may mediate rictor-mTOR signaling to downstream targets including PKCα (7). Furthermore, the rictor-mTOR complex has been identified as the previously elusive PDK2 responsible for the phosphorylation of Akt/PKB on Ser473, facilitating phosphorylation of Akt/PKB on Thr308 by PDK1 and required for the full activation of Akt/PKB (8).Recently raptor has been identified as a direct substrate of the AMP-activated protein kinase (AMPK) (9). AMPK phosphorylates raptor on Ser722/Ser792 (9). This phosphorylation is essential for inhibition of the raptor-containing mTOR complex 1 (mTORC1) and induces cell cycle arrest when cells are stressed for energy (9). These findings suggest that raptor is a critical switch that correlates cell cycle progression with energy status.

  1. Hara, K. et al. (2002) Cell 110, 177-189.
  2. Kim, D. et al. (2002) Cell 110, 163-175.
  3. Beugnet, A. et al. (2003) J. Biol. Chem. 278, 40717-40722.
  4. Nojima, H. et al. (2003) J. Biol. Chem. 278, 15461-15464.
  5. Oshiro, N. et al. (2004) Genes Cells 9, 359-366.
  6. Kim, D. H. et al. (2003) Mol. Cell 11, 895-904.
  7. Sarbassov, D. et al. (2004) Curr. Biol. 14, 1296-1302.
  8. Sarbassov, D.D. et al. (2005) Science 307, 1098-1101.
  9. Gwinn, D.M. et al. (2008) Mol Cell 30, 214-26.

Application References

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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