Product Pathways - DNA Damage
Phospho-p63 (Ser160/162) Antibody #4981
|W IHC-P F||H (M) (R) (C) (X)||Endogenous||75||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat C=Chicken X=Xenopus
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-p63 (Ser160/162) Antibody detects endogenous levels of p63 only when phosphorylated at serine 160/162.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser160/160 of human TAp63-alpha. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from untreated or nocodazole-treated (50 ng/ml, 24 h) ME-180 cells using Phospho-p63 (Ser160/162) Antibody (upper) or p63alpha Antibody #4892 (lower).
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the esophagus, showing nuclear localization, using Phospho-p63 (Ser160/162) Antibody.
Immunohistochemical analysis of paraffin-embedded ME-180 cells, untreated (left) or nocodozole-treated (right), showing induced nuclear staining, using Phospho-p63 (Ser160/162) Antibody.
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma, using Phospho-p63 (Ser160/162) Antibody in the presence of control peptide (left) or antigen-specific peptide (right).
The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Amplification of the p63 gene is frequently observed in squamous cell carcinomas of the lung, head and neck (2,3). The p63 gene contains an alternative transcription intiation site that yields a 40 kDa deltaNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the alpha, beta and gamma isoforms (3,4).
TAp63-alpha (full-length) contains multiple serine residues followed by proline (Ser-Pro motif) that are potential cdk substrates expected to be phosphorylated in mitosis. Among these are Ser160, Ser162, Ser395, and Ser455.
- Levine, A. J. et al. (1997) Cell 88, 323-331.
- Waltermann, A. et al. (2003) Oncogene 22, 5686-5693.
- Hibi, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 5462-5467.
- Yang, A. et al. (1999) Nature 398, 714-718.
- Westfall, M. D. et al. (2005) Ultraviolet Radiation Induces Phosphorylation and Ubiquitin-Mediated Degradation of deltaNp63alpha. Cell Cycle 4 (5), 710-716. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.