Product Pathways - NF-kB Signaling
RIP2 Antibody #4982
PhosphoSitePlus® protein, site, and accession data: RIPK2
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 62 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4982:
- Western Blotting
Specificity / Sensitivity
RIP2 Antibody detects endogenous levels of total RIP2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human RIP2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293T cells transfected with RIP2-ΔCARD, and extracts from K562 and MDA-MB-468 cells, using RIP2 Antibody.
Background
Receptor Interacting Protein 2 (RIP2) is a serine/threonine kinase with a carboxy-terminal caspase activation and recruitment domain (CARD). Association of RIP2 with the tumor necrosis factor receptor (TNFR) causes activation of NF-kB and induction of apoptosis (1-3). Expression of RIP2 is induced in macrophages upon exposure to bacterial cell wall components such as LPS. RIP2-deficient mouse models demonstrate that this kinase integrates and transduces signals for both the innate and adaptive immune system (4,5).
- Inohara, N. et al. (1998) J. Biol. Chem. 273, 12296-12300.
- McCarthy, J.V. et al. (1998) J. Biol. Chem. 273, 16968-16975.
- Thome, M. et al. (1998) Curr. Biol. 8, 885-888.
- Chin, A.I. et al. (2002) Nature 416, 190-194.
- Kobayashi, K. et al. (2002) Nature 416, 194-199.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
