Cell Signaling Technology

Product Pathways - NF-kB Signaling

MAVS Antibody (Rodent Specific) #4983

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC M R Endogenous 75, 52 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MAVS Antibody (Rodent Specific) detects endogenous levels of total MAVS/VISA protein. The bands detected at 52 and 75 kDa correlate with those described by Seth et al. (2005).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys140 of mouse MAVS/VISA. Antibodies were purified by affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from A20 and CTLL-2 cell lines using MAVS Antibody (Rodent Specific).

IF-IC

IF-IC

Confocal immunofluorescent analysis of an A20 cell using MAVS Antibody (Rodent Specific) (green) showing colocalization with mitochondria that have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Background

The mitochondrial antiviral signaling protein (MAVS, VISA) contributes to innate immunity by triggering IRF-3 and NF-κB activation in response to viral infection, leading to the production of IFN-β (1). The MAVS protein contains an N-terminal CARD domain and a C-terminal mitochondrial transmembrane domain. The MAVS adaptor protein plays a critical and specific role in viral defenses (2). MAVS acts downstream of the RIG-I RNA helicase and viral RNA sensor, leading to the recruitment of IKKε, TRIF and TRAF6 (3,4). Some viruses have evolved strategies to circumvent these innate defenses by using proteases that cleave MAVS to prevent its mitochondrial localization (5,6).

  1. Seth, R.B. et al. (2005) Cell 122, 669-682.
  2. Sun, Q. et al. (2006) Immunity 24, 633-642.
  3. Xu, L.G. et al. (2005) Mol. Cell 19, 727-740.
  4. Yoneyama, M. et al. (2004) Nat. Immunol. 5, 730-737.
  5. Lin, R. et al. (2006) J. Virol. 80, 6072-6083.
  6. Chen, Z. et al. (2007) J. Virol. 81, 964-976.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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