Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Tec Antibody #4987

Applications Reactivity Sensitivity MW (kDa) Source
W H M Endogenous 58 to 75 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Tec Antibody detects endogenous levels of Tec protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding amino acid residue 110 of human Tec. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using Tec Antibody.

Background

Tec kinase belongs to a structurally related subfamily of protein tyrosine kinases (PTKs) that includes Btk, Itk (also known as Emt or Tsk), Bmx, and Txk (or Rlk) (1). With the exception of Txk, the members of this subfamily possess a long amino-terminal region consisting of a pleckstrin homology (PH) domain and a Tec homology (TH) domain . Because PH domains bind phosphoinositides with high affinity, the Tec family kinases have been proposed to act downstream of phosphatidylinositol 3-kinase (PI3-kinase) in signaling pathways. Binding of the PH domain with phosphoinositides is probably required for targeting of Tec family kinases to the cell membrane (2). Tec kinase is activated in response to many upstream signaling events including antigen receptor, RTK, GPCR, and integrin stimulation (3,4). Activated Tec kinase directly phosphorylates substrates such as PLC-gamma 2 and BRDG1 docking protein (5) and mediates downstream signaling.

  1. August, A. et al. (2002) Int. J. Biochem. Cell Biol. 34, 1184-1189.
  2. Pillai, S. and Moran, S.T. (2002) Biochim. Biophys. Acta. 1602, 162-167.
  3. Lewis, C. M. et al. (2001) Curr. Opin. Immunol. 13, 317-325.
  4. Schmidt, U. et al. (2004) Int. Arch. Allergy Immunol. 134, 65-78.
  5. Ohya, K. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 11976-11981.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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