Cell Signaling Technology

Product Pathways - Translational Control

PABP1 Antibody #4992

Applications Reactivity Sensitivity MW (kDa) Source
W IHC-P H M R Mk (X) (Z) (B) Endogenous 71 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  X=Xenopus  Z=Zebrafish  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PABP1 Antibody detects endogenous levels of total PABP1 and PABP3 proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids at the amino-terminus of human PABP1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29 cells, using PABP1 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using PABP1 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PABP1 Antibody.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using PABP1 Antibody in the presence of control peptide (left) or antigen-specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using PABP1 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, using PABP1 Antibody.


Background

Poly(A)-binding protein 1 (PABP1) associates with the 3' poly(A) tail of mRNA and also eIF4F (1,2). eIF4F is a complex whose functions include the recognition of the mRNA 5' cap structure (eIF4E), delivery of an RNA helicase to the 5' region (eIF4A), bridging of the mRNA and the ribosome (eIF4G), and circularization of the mRNA via interaction between eIF4G and the poly(A) binding protein (PABP). PABP1 has been shown to have multiple functions including translation initiation, mRNA stabilization, and mRNA turnover (3,4). Phosphorylation of PABP has been shown to enhance RNA binding in eukaryotes, and PABP1 has been shown to shuttle between the nucleus and cytoplasm (5,6). PABP1 is methylated on Arg455 and Arg460 by the CARM1 protein methyltransferase (7,8); however, the function of this methylation has yet to be determined.

  1. Sachs, A. B. et al. (1986) Cell 45, 827-835.
  2. Piron, M. et al. (1998) EMBO J. 17, 5811-5821.
  3. Caponigro, G. and Parker, R. (1995) Genes Dev. 9, 2421-2432.
  4. Sachs, A.B. and Davis, R.W. (1989) Cell 58, 857-867.
  5. Le, H. et al. (2000) J. Biol. Chem. 275, 17452-17462.
  6. Afonina, E. et al. (1998) J. Biol. Chem. 273, 13015-13021.
  7. Lee, J. and Bedford, M.T. (2002) EMBO Rep 3, 268-73.
  8. Yadav, N. et al. (2003) Proc Natl Acad Sci U S A 100, 6464-8.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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