Product Pathways - Protein Folding/Stability
UBC3 Antibody #4997
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W F | H M R | Endogenous | 32 | Rabbit |
Applications Key:
W=Western Blotting
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
UBC3 Antibody detects endogenous levels of total UBC3 and UBC3B protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human UBC3. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Jurkat, CAD and C6 cells, using UBC3 Antibody.
Flow Cytometry
Flow cytometric analysis of untreated Jurkat cells, using UBC3 antibody (blue) compared to a nonspecific negative control antibody (red).
Background
Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). Recent data suggest that activated E2 associates transiently with E3, and that the dissociation is a critical step for ubiqitination (5). UBC3, the mammalian orthologue of yeast Cdc34, and UBC3B, a UBC3 family member, are E2 ubiquitin-carrier proteins. These proteins contain a conserved core domain containing a cysteine residue, which forms the thioester bond with ubiquitin (6). UBC3 in concert with the SCFSkp2 (Skp1, Cullin and F-box protein/Skp2) complex mediates cell cycle progression from G1 to S phase by targeting the CDK inhibitor p27 for proteolysis (7). UBC3B in concert with the SCFb-Trcp (Skp1, Cullin and F-box protein/b-Trcp) complex mediates degradation of b-catenin (6).
- Ciechanover, A. (1998) EMBO J. 17, 7151-7160.
- Hochstrasser, M. (2000) Nat. Cell Biol. 2, E153-E157.
- Hochstrasser, M. (2000) Science 289, 563-564.
- DeSalle, L.M. and Pagano, M. (2001) FEBS Lett. 490, 179-189.
- Deffenbaugh, A. E. et al. (2003) Cell 114, 611-622.
- Semplici, F. et al. (2002) Oncogene 21 , 3978-3987 .
- Pagano, M. et al. (1995) Science 269, 682-685.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 4996 UBC3B Antibody
- 3936 Ubiquitin (P4D1) Mouse mAb
- 4995 CUL1 Antibody
- 2552 p27 Kip1 Antibody
- 9561 Phospho-β-Catenin (Ser33/37/Thr41) Antibody
- 9562 β-Catenin Antibody
- 9564 Phospho-β-Catenin (Ser45) Antibody
- 9565 Phospho-β-Catenin (Thr41/Ser45) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
