Product Pathways - PI3K / Akt Signaling
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Biotinylated) #5012
|W F||H M R Hm Mk Dm Z B (C) (X) (Dg) (Pg)||Endogenous||60||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey C=Chicken Dm=D. melanogaster X=Xenopus Z=Zebrafish B=Bovine Dg=Dog Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb detects endogenous levels of Akt only when phosphorylated at Ser473.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser473 of human Akt.
Western blot analysis of extracts of Jurkat cells, treated with either Calyculin A #9902 or LY294002 #9901, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Biotinylated) #5012 and detected with Streptavidin-HRP #3999.
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 #9903 treated (blue), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Biotinylated) compared to XP® Rabbit (DA1E) mAb IgG Isotype Control (Biotinylated) #4096 (red).
This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 reacts with human, mouse, rat, Drosophila melanogaster, hamster, bovine and zebrafish phospho-Akt (Ser473) protein. CST expects that Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Biotinylated) will also recognize phospho-Akt (Ser473) in these species.
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip1 (15) and p21 Waf1/Cip1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).
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- 4060 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb
- 4071 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate)
- 4075 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 3999 Streptavidin-HRP
- 3419 Streptavidin (Sepharose Bead Conjugate)
- 7727 Biotinylated Protein Ladder Detection Pack
- 4691 Akt (pan) (C67E7) Rabbit mAb
For Research Use Only. Not For Use In Diagnostic Procedures.