Product Pathways - MAPK Signaling
p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Biotinylated) #5013
|W IP F||H M R Mk Mi Dm Z B Pg||Endogenous||42, 44||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Mi=Mink Dm=D. melanogaster Z=Zebrafish B=Bovine Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Biotinylated) detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. The antibody does not cross-react with JNK/SAPK or p38 MAP kinase.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the C-terminus of rat p44 MAP kinase.
Western blot analysis of extracts from various cell lines using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Biotinylated).
This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 reacts with human, mouse, rat, monkey, mink, pig, Saccharomyces cerevisiae, Drosophila melanogaster, hamster, bovine and zebrafish p44/42 MAPK protein. CST expects that p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Biotinylated) will also recognize MAPK in these species.
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059.
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- Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310.
- Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74.
- Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
- Marais, R. et al. (1993) Cell 73, 381-93.
- Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.
- Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.
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- 4695 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb
- 4370 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb
- 9194 p44/42 MAPK (Erk1/2) Control Cell Extracts
- 3419 Streptavidin (Sepharose Bead Conjugate)
- 3999 Streptavidin-HRP
- 7727 Biotinylated Protein Ladder Detection Pack
Rabbit monoclonal antibody is produced Produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and U.S.S.N. 11/476,277) from Epitomics, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.