Cell Signaling Technology

Product Pathways - Metabolism

GAPDH (14C10) Rabbit mAb (Biotinylated) #5014

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC F H M R Mk Endogenous 37 Rabbit IgG

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GAPDH (14C10) Rabbit mAb (Biotinylated) detects endogenous levels of total GAPDH protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide near the carboxy terminus of human GAPDH.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells using GAPDH (14C10) Rabbit mAb (Biotinylated).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, using GAPDH (14C10) Rabbit mAb (Biotinylated) (blue) compared to XP® Rabbit (DA1E) mAb IgG Isotype Control (Biotinylated) #4096 (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF-7 cells using GAPDH (14C10) Rabbit mAb (Biotinylated) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Description

This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated GAPDH (14C10) Rabbit mAb #2118 reacts with human, mouse, rat and monkey GAPDH. CST expects that GAPDH (14C10) Rabbit mAb (Biotinylated) will also recognize GAPDH in these species.

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5).

  1. Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
  2. Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
  3. Zheng, L. et al. (2003) Cell 114, 255-66.
  4. Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
  5. Wang, Q. et al. (2005) FASEB J. 19, 869-71.

Application References

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Companion Products

Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and U.S.S.N. 11/476,277) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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