Product Pathways - Metabolism
GAPDH (14C10) Rabbit mAb (Biotinylated) #5014
|5014S||100 µl (10 western blots)||---||In Stock||---|
|5014||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||37||Rabbit IgG|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
GAPDH (14C10) Rabbit mAb (Biotinylated) detects endogenous levels of total GAPDH protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide near the carboxy terminus of human GAPDH.
Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells using GAPDH (14C10) Rabbit mAb (Biotinylated).
Flow cytometric analysis of HeLa cells, using GAPDH (14C10) Rabbit mAb (Biotinylated) (blue) compared to XP® Rabbit (DA1E) mAb IgG Isotype Control (Biotinylated) #4096 (red).
Confocal immunofluorescent analysis of MCF-7 cells using GAPDH (14C10) Rabbit mAb (Biotinylated) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated GAPDH (14C10) Rabbit mAb #2118 reacts with human, mouse, rat and monkey GAPDH. CST expects that GAPDH (14C10) Rabbit mAb (Biotinylated) will also recognize GAPDH in these species.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
DRAQ5® is a registered trademark of Biostatus Limited.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.