Cell Signaling Technology

Product Pathways - Companion Products

HA-Tag (C29F4) Rabbit mAb (Biotinylated) #5017

Applications Reactivity Sensitivity Isotype
W F All Transfected Only Rabbit IgG

Applications Key:  W=Western Blotting  F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Protocols

Specificity / Sensitivity

HA-Tag (C29F4) Rabbit mAb (Biotinylated) detects exogenously expressed proteins containing the HA epitope tag.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA).

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected or transfected with a construct overexpressing HA-Akt, using HA-Tag (C29F4) Rabbit mAb (Biotinylated). Detection was performed with Streptavidin-HRP followed by LumiGlo® and Peroxidase #7003.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of COS cells, untransfected (blue) or transfected with HA-Tag (green), using HA-Tag (C29F4) Rabbit mAb (Biotinylated).

Description

This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions and tested using COS cells transfected with HA-tagged protein.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

The HA tag is derived from an epitope of the influenza hemagglutinin protein which has been used extensively as a general epitope tag in expression vectors (1).

  1. Field, J. et al. (1988) Mol Cell Biol 8, 2159-65.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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