Product Pathways - Companion Products
HA-Tag (C29F4) Rabbit mAb (Biotinylated) #5017
|W F||All||Transfected Only||Rabbit IgG|
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Specificity / Sensitivity
HA-Tag (C29F4) Rabbit mAb (Biotinylated) detects exogenously expressed proteins containing the HA epitope tag.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA).
Western blot analysis of extracts from COS cells, untransfected or transfected with a construct overexpressing HA-Akt, using HA-Tag (C29F4) Rabbit mAb (Biotinylated). Detection was performed with Streptavidin-HRP followed by LumiGlo® and Peroxidase #7003.
This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions and tested using COS cells transfected with HA-tagged protein.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The HA tag is derived from an epitope of the influenza hemagglutinin protein which has been used extensively as a general epitope tag in expression vectors (1).
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- 3999 Streptavidin-HRP
- 3724 HA-Tag (C29F4) Rabbit mAb
- 2367 HA-Tag (6E2) Mouse mAb
- 2999 HA-Tag (6E2) Mouse mAb (HRP Conjugate)
- 2350 HA-Tag (6E2) Mouse mAb (Alexa Fluor® 488 Conjugate)
- 3444 HA-Tag (6E2) Mouse mAb (Alexa Fluor® 647 Conjugate)
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.