HA-Tag (C29F4) Rabbit mAb (Biotinylated) #5017
|5017S||100 µl (10 western blots)||---||In Stock||---|
|5017||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Transfected Only||Rabbit IgG|
Applications Key: W=Western Blotting, F=Flow Cytometry
Specificity / Sensitivity
HA-Tag (C29F4) Rabbit mAb (Biotinylated) detects exogenously expressed proteins containing the HA epitope tag.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA).
Western blot analysis of extracts from COS cells, untransfected or transfected with a construct overexpressing HA-Akt, using HA-Tag (C29F4) Rabbit mAb (Biotinylated). Detection was performed with Streptavidin-HRP followed by LumiGlo® and Peroxidase #7003.
This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions and tested using COS cells transfected with HA-tagged protein.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The HA tag is derived from an epitope of the influenza hemagglutinin protein which has been used extensively as a general epitope tag in expression vectors (1).
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For Research Use Only. Not For Use In Diagnostic Procedures.
LumiGLO® is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patent No. 5,675,063) from Epitomics, Inc.