Cell Signaling Technology

Product Pathways - Chromatin Regulation

ASH2L (D93F6) XP™ Rabbit mAb #5019

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M R Mk (Dm) Endogenous 80, 65 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Dm=D. melanogaster
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

ASH2L (D93F6) XP™ Rabbit mAb detects endogenous levels of all known isoforms of the ASH2L protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human ASH2L protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ASH2L (D93F6) XP™ Rabbit mAb.

IF-IC

IF-IC

Confocal imunofluorescent analysis of HeLa cells using ASH2L (D93F6) XP™ Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Background

The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 on Lys4 and functions as a transcriptional co-activator (1). While yeast contain only one known Set1 protein, mammals contain six Set1-related proteins: SET1A, SET1B, MLL1, MLL2, MLL3 and MLL4, all of which methylate histone H3 on Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common structural subunits WDR5, RBBP5 and ASH2L (2-6). Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 on Lys4 (2-6). MLL translocations are found in a large number of hematological malignancies, suggesting that Set1 histone methyltransferase complexes play a critical role in leukemogenesis (6).

  1. Miller, T. et al. (2001) Proc Natl Acad Sci USA 98, 12902-7.
  2. Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.
  3. Tenney, K. and Shilatifard, A. (2005) J Cell Biochem 95, 429-36.
  4. Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31.
  5. Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.
  6. Hughes, C.M. et al. (2004) Mol Cell 13, 587-97.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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