Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Myosin IIa (Ser1943) Antibody #5026

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 230 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Myosin IIa (Ser1943) Antibody detects endogenous levels of myosin IIa protein only when phosphorylated at Ser1943.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1943 of human myosin IIa protein. Antibodies are purified using protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, A-431 and 293T cells, untreated or treated with λ phosphatase and calf intestinal phosphatase (CIP), using Phospho-Myosin IIa (Ser1943) Antibody (upper) or Myosin IIa Antibody #3403 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 cells, untreated or treated with λ phosphatase and calf intestinal phosphatase (CIP), using Phospho-Myosin IIa (Ser1943) Antibody. The antibody was pre-incubated with peptides corresponding to unphosphorylated Myosin IIa or Myosin IIa phosphorylated at Ser1943, as indicated.

Background

Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Research studies have shown that phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7).

  1. Conti, M.A. and Adelstein, R.S. (2008) J Cell Sci 121, 11-18.
  2. Sandquist, J.C. et al. (2006) J Biol Chem 281, 35873-83.
  3. Even-Ram, S. et al. (2007) Nat Cell Biol 9, 299-309.
  4. Vicente-Manzanares, M. et al. (2007) J Cell Biol 176, 573-80.
  5. Wylie, S.R. and Chantler, P.D. (2008) Mol Biol Cell 19, 3956-68.
  6. Sandquist, J.C. and Means, A.R. (2008) Mol Biol Cell 19, 5156-67.
  7. Dulyaninova, N.G. et al. (2007) Mol Biol Cell 18, 3144-55.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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