Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - MAPK Signaling

MEF2C (D80C1) XP® Rabbit mAb #5030

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M Endogenous 50-60 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MEF2C (D80C1) XP® Rabbit mAb detects endogenous levels of total MEF2C protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Met182 of human MEF2C protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cells lines using MEF2C (D80C1) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of C2C12 cells, undifferentiated (left) or differentiated for 3 days (right), using MEF2C (D80C1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Background

MEF2C is a member of the MEF2 (myocyte enhancer factor 2) family of transcription factors. In mammals, there are four MEF2C-related genes (MEF2A, MEF2B, MEF2C and MEF2D) that encode proteins that exhibit significant amino acid sequence similarity within their DNA binding domains and, to a lesser extent, throughout the rest of the proteins (1). The MEF2 family members were originally described as muscle-specific DNA binding proteins that recognize MEF2 motifs found within the promoters of many muscle-specific genes (2,3). Recently, several groups have reported MEF2 binding activity and MEF2 proteins in a wide variety of cell types where these proteins appear to play an important role in growth factor- and stress-induced early gene responses (4-6).

  1. Shore, P. et al. (1995) Eur. J. Biochem. 229, 1-13.
  2. Martin, J. F. et al. (1994) Mol. Cell. Biol. 14, 1647-1656.
  3. Yu, Y. T. et al. (1992) Genes Dev. 6, 1783-1798.
  4. Han, J. et al. (1997) Nature 386, 296-299.
  5. Kato, Y. et al. (1997) EMBO J. 16, 7054-7066.
  6. Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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