Cell Signaling Technology

Product Pathways - Translational Control

La Antigen (D19B3) Rabbit mAb #5034

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 50 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

La Antigen (D19B3) Rabbit mAb detects endogenous levels of total La Antigen protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu97 of human La Antigen protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using La Antigen (D19B3) Rabbit mAb.

Background

La antigen is recognized by antibodies in patients with autoimmune disorders such as systemic lupus erythematosus and Sjögren's syndrome (1). La antigen binds to the 5'-noncoding region of poliovirus RNA and is an IRES trans-acting factor (1,2). Depletion of La antigen reduces the function of poliovirus IRES in vivo (3). La antigen, when phosphorylated at Ser366, has been shown to associate with nuclear precursor tRNAs and facilitate their processing (4). The nonphosphorylated La antigen interacts with the mRNAs that have 5'-terminal oligopyrimidine (5'TOP) motifs to control protein synthesis (4).

  1. Meerovitch, K. et al. (1993) J Virol 67, 3798-807.
  2. Meerovitch, K. et al. (1989) Genes Dev 3, 1026-34.
  3. Costa-Mattioli, M. et al. (2004) Mol Cell Biol 24, 6861-70.
  4. Intine, R.V. et al. (2003) Mol Cell 12, 1301-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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