Cell Signaling Technology

Product Pathways - Metabolism

SGLT1 Antibody #5042

Applications Reactivity Sensitivity MW (kDa) Source
W H R Endogenous 61 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SGLT1 Antibody detects endogenous levels of total SGLT1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly247 of human SGLT1 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Hep G2 and 293T cells using SGLT1 Antibody.

Background

Sodium/glucose cotransporter 1 (SGLT1) is an active glucose transporter, which utilizes sodium gradients to transport glucose into cells independent of extracellular glucose concentration. SGLT1 is an essential glucose active transport protein that helps maintain high intracellular glucose levels (1). Expression of SGLT1 is mainly seen in intestinal and kidney epithelial cells, although a recent study also characterized SGLT1 expression in cardiac myocytes (2). Abnormal SGLT1 expression may be associated with cases of type 2 diabetes mellitus and myocardial ischaemia (2). Mutation of the corresponding SGLT1 gene can result in congenital glucose/galactose malabsorption, which can lead to neonatal diarrhea and subsequent death if left untreated (3). A recent study of the role of EGFR in cancer cell survival indicates that EGFR can prevent autophagic cell death independent of EGFR kinase activity because the receptor interacts with and stabilizes SLGT1 to maintain basal intracellular glucose levels (4).

  1. Wright, E.M. et al. (1994) J Exp Biol 196, 197-212.
  2. Banerjee, S.K. et al. (2009) Cardiovasc Res 84, 111-8.
  3. Martín, M.G. et al. (1996) Nat Genet 12, 216-20.
  4. Weihua, Z. et al. (2008) Cancer Cell 13, 385-93.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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