Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Stem Cell and Lineage Markers

Sox2 (D6D9) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #5049

Applications Reactivity Sensitivity Isotype
IF-IC F H (Mk) (B) (Dg) (Hr) Endogenous Rabbit IgG

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  Mk=Monkey  B=Bovine  Dg=Dog  Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Sox2 (D6D9) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total Sox2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly179 of human Sox2 protein.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and NTERA-2 cells (green) using Sox2 (D6D9) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate).

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 (left) and HeLa (right) cells using Sox2 (D6D9) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Sox2 (D6D9) XP® Rabbit mAb #3579.

Background

Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4, and Nanog cooperate in this network (1-3), and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8).

  1. Nichols, J. et al. (1998) Cell 95, 379-391.
  2. Avilion, A.A. et al. (2003) Genes Dev. 17, 126-140.
  3. Rodda, D.J. et al. (2005) J. Biol. Chem. 280, 24731-24737.
  4. Matin, M.M. et al. (2004) Stem Cells 22, 659-668.
  5. Niwa, H. et al. (2000) Nat. Genet. 24, 372-376.
  6. Boyer, L.A. et al. (2005) Cell 122, 947-956.
  7. Loh, Y.H. et al. (2006) Nat. Genet. 38, 431-440.
  8. Bass, A.J. et al. (2009) Nat Genet 41, 1238-42.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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