Cell Signaling Technology

Product Pathways - Protein Folding

PDI (C81H6) Rabbit mAb (Alexa Fluor® 488 Conjugate) #5051

Applications Reactivity Sensitivity Isotype
IF-IC H M R Mk Endogenous Rabbit

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

PDI (C81H6) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total PDI protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PDI.

IF-IC

IF-IC

Immunofluorescent analysis of NIH/3T3 cells using PDI (C81H6) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) and a β-Actin Mouse mAb (Alexa Fluor® 594 Conjugate) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated PDI (C81H6) Rabbit mAb #3501.

Background

During their synthesis, secretory proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Protein disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes the ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation in folding proteins (3).

  1. Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-148.
  2. Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep. 6, 28-32.
  3. Tu, B.P. and Weissman, J.S. (2004) J. Cell Biol. 164, 341-346.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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