Product Pathways - Protein Translation
Argonaute 3 (D15D2) Rabbit mAb #5054
|5054S||100 µl (10 western blots)||---||In Stock||---|
|5054||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Monkey||Endogenous||97||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Rat.
Specificity / Sensitivity
Argonaute 3 (D15D2) Rabbit mAb recognizes endogenous levels of total Argonaute 3 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val160 of human Argonaute 3 protein.
Western blot analysis of extracts from PANC-1, HeLa, and C2C12 cells using Argonaute 3 (D15D2) Rabbit mAb.
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human Argonaute 3 (hArgonaute 3-DDK; +), using Argonaute 3 (D15D2) Rabbit mAb (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Small non-coding RNAs are important regulators of gene expression in higher eukaryotes (1,2). Several classes of small RNAs, including short interfering RNAs (siRNAs) (3), microRNAs (miRNAs) (4), and Piwi-interacting RNAs (piRNAs) (5), have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation, and stress response (1,2). MicroRNAs regulate gene expression by modulating mRNA translation or stability (2). MicroRNAs function together with the protein components in the complexes called micro-ribonucleoproteins (miRNPs) (2). Among the most important components in these complexes are Argonaute proteins (1,2). There are four members in the mammalian Argonaute family and only Argonaute 2 (Ago2) possesses the Slicer endonuclease activity (1,2). Argonaute proteins participate in the various steps of microRNA-mediated gene silencing, such as repression of translation and mRNA turnover (1).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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