Cell Signaling Technology

Product Pathways - Translational Control

Argonaute 3 (D15D2) Rabbit mAb #5054

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M Mk (R) Endogenous 97 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Argonaute 3 (D15D2) Rabbit mAb recognizes endogenous levels of total Argonaute 3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val160 of human Argonaute 3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from PANC-1, HeLa, and C2C12 cells using Argonaute 3 (D15D2) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human Argonaute 3 (hArgonaute 3-DDK; +), using Argonaute 3 (D15D2) Rabbit mAb (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

Small non-coding RNAs are important regulators of gene expression in higher eukaryotes (1,2). Several classes of small RNAs, including short interfering RNAs (siRNAs) (3), microRNAs (miRNAs) (4), and Piwi-interacting RNAs (piRNAs) (5), have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation, and stress response (1,2). MicroRNAs regulate gene expression by modulating mRNA translation or stability (2). MicroRNAs function together with the protein components in the complexes called micro-ribonucleoproteins (miRNPs) (2). Among the most important components in these complexes are Argonaute proteins (1,2). There are four members in the mammalian Argonaute family and only Argonaute 2 (Ago2) possesses the Slicer endonuclease activity (1,2). Argonaute proteins participate in the various steps of microRNA-mediated gene silencing, such as repression of translation and mRNA turnover (1).

  1. Peters, L. and Meister, G. (2007) Mol Cell 26, 611-23.
  2. Filipowicz, W. et al. (2008) Nat Rev Genet 9, 102-14.
  3. Caplen, N.J. et al. (2001) Proc Natl Acad Sci USA 98, 9742-7.
  4. Mourelatos, Z. et al. (2002) Genes Dev 16, 720-8.
  5. Girard, A. et al. (2006) Nature 442, 199-202.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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