Cell Signaling Technology

Product Pathways - Metabolism

NPC1L1 Antibody #5058

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 140 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NPC1L1 Antibody detects endogenous levels of total NPC1L1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to central residues of human NPC1L1 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Hep G2 and MKN-45 cells using NPC1L1 Antibody.

Background

Niemann-Pick C1-like 1 (NPC1L1) is a transmembrane protein that plays a critical role in cholesterol absorption (1). It is highly expressed in small intestine and localized along the brush border in both human and mouse epithelial cells (2,3). NPC1L1 mediates cholesterol uptake via vesicular endocytosis (4). Ezetimibe, a potent cholesterol absorption inhibitor used to treat hypercholesterolemia (5), inhibits cholesterol uptake by preventing NPC1L1 from incorporating into clathrin-coated vesicles (4).

  1. Altmann, S.W. et al. (2004) Science 303, 1201-4.
  2. Davis, H.R. et al. (2004) J Biol Chem 279, 33586-92.
  3. Sané, A.T. et al. (2006) J Lipid Res 47, 2112-20.
  4. Ge, L. et al. (2008) Cell Metab 7, 508-19.
  5. Davis, H.R. and Veltri, E.P. (2007) J Atheroscler Thromb 14, 99-108.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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