Cell Signaling Technology

Product Pathways - Protein Folding

HO-1 (P249) Antibody #5061

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 28 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

HO-1 (P249) Antibody detects endogenous levels of total HO-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid residues surrounding Pro249 of human HO-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or arsenite-treated, using HO-1 (P249) Antibody.

Background

Heme oxygenase (HO) is the rate-limiting enzyme in the catabolism of heme that results in the release of carbon monoxide, iron, and biliverdin (1). The products of this enzymatic reaction play important biological roles in antioxidant, anti-inflammatory and cytoprotective functions (2). Heme oxygenase comprises two isozymes, including the constitutively expressed HO-2 isozyme and the inducible HO-1 isozyme (3). Inducible HO-1 is expressed as an adaptive response to several stimuli, including heme, metals, and hormones (4). The induction of HO-1 has been implicated in numerous disease states, such as transplant rejection, hypertension, atherosclerosis, Alzheimer disease, endotoxic shock, diabetes, inflammation, and neurological disorders (1,5).

  1. Abraham, N.G. and Kappas, A. (2008) Pharmacol Rev 60, 79-127.
  2. Otterbein, L.E. et al. (2003) Trends Immunol 24, 449-55.
  3. Cruse, I. and Maines, M.D. (1988) J Biol Chem 263, 3348-53.
  4. Maines, M.D. (1988) FASEB J 2, 2557-68.
  5. Schipper, H.M. et al. (2009) Curr Alzheimer Res 6, 424-30.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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