Product Pathways - Cytoskeletal Signaling
α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 488 Conjugate) #5063
PhosphoSitePlus® protein, site, and accession data: TUBA1B
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC F | H M R Mk Dm B (Dg) | Endogenous | Rabbit IgG |
Applications Key:
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Dm=D. melanogaster
B=Bovine
Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5063:
- Flow, Immunofluorescence
Specificity / Sensitivity
α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total α-tubulin protein and does not cross-react with recombinant β-tubulin.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the amino terminus of human α-tubulin protein.
Flow Cytometry
Flow cytometric analysis of HeLa cells using α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) and Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) #2975 (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 488 Conjugate (green). Red = propidium iodide (fluorescent DNA dye).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated α-Tubulin (11H10) Rabbit mAb #2125.
Background
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Application References
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Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
