Product Pathways - Apoptosis
Apaf-1 (R205) Antibody #5088
PhosphoSitePlus® protein, site, and accession data: APAF
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H (Mk) | Endogenous | 135 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Apaf-1 (R205) Antibody detects endogenous levels of total Apaf-1 protein.
Source / Purification
Polyclonal antibodies are prepared by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg205 of human Apaf-1 protein. Antibodies were purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from THP-1, ACHN, and 293 cells using Apaf-1 (R205) Antibody.
Background
Apoptotic protease activating factor 1 (Apaf-1), originally identified as the mammalian homolog of the C. elegans apoptotic regulatory protein CED-4, is an important signaling protein involved in the activation of caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms a complex with caspase-9 in the presence of cytochrome c and dATP, ultimately leading to caspase-9 activation and subsequent activation of caspase-3 (2,3). The protein contains an amino-terminal CARD domain, a central CED-4 homology domain, and multiple WD-40 repeats at the carboxy-terminus. Several isoforms of Apaf-1 are expressed through alternative splicing generating a small insert following the CARD domain as well as an extra WD-40 repeat (4). Apaf-1 knock-out mice display widespread defects in apoptosis and resistance to a variety of apoptotic stimuli (5,6).
- Zou, H. et al. (1997) Cell 90, 405-13.
- Zou, H. et al. (1999) J Biol Chem 274, 11549-56.
- Saleh, A. et al. (1999) J Biol Chem 274, 17941-5.
- Benedict, M.A. et al. (2000) J Biol Chem 275, 8461-8.
- Cecconi, F. et al. (1998) Cell 94, 727-37.
- Yoshida, H. et al. (1998) Cell 94, 739-50.
Application References
- Zhu, J. et al. (2011) PLoS One 6, e23720. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.
