Product Pathways - PI3K / Akt Signaling
Phospho-GSK-3α (Ser21) (27E5) Mouse mAb #5090
PhosphoSitePlus® protein, site, and accession data: GSK3A
| No. | Ordering | |
|---|---|---|
| 5090 | carrier free & custom formulation / quantity | email request |
| Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|
| E-P | All | 51 | Mouse IgG2b |
Applications Key:
E-P=ELISA (Peptide)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
- 5090:
- ELISA Peptide
Specificity / Sensitivity
Phospho-GSK-3alpha (Ser21) (27E5) Monoclonal Antibody can be used in high throughput kinase assays and drug discovery applications. It detects peptides derived from GSK-3alpha phosphorylated at Ser21.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser21 of human GSK-3alpha. Antibody is purified using protein G affinity chromatography.
Description
This antibody is formulated in PBS (no BSA/no glycerol) and quality controlled for use in ELISA and other drug discovery applications. This is a sample antibody and intended for use by drug discovery scientists.
Background
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
- Welsh, G.I. et al. (1996) Trends Cell. Biol. 6, 274-279.
- Srivastava, A.K. and Pandey, S.K. (1998) Mol. Cell. Biochem. 182, 135-141.
- Cross, D.A. et al. (1995) Nature 378, 785-789.
- Nusse, R. (1997) Cell 89, 321-323.
- Diehl, J.A. et al. (1998) Genes Dev. 12, 3499-3511.
Application References
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Companion Products
For Research Use Only. Not For Use In Diagnostic Procedures.