Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-GSK-3α (Ser21) (27E5) Mouse mAb #5090

Applications Reactivity MW (kDa) Isotype
E-P All 51 Mouse IgG2b

Applications Key:  E-P=ELISA (Peptide)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Protocols

Specificity / Sensitivity

Phospho-GSK-3alpha (Ser21) (27E5) Monoclonal Antibody can be used in high throughput kinase assays and drug discovery applications. It detects peptides derived from GSK-3alpha phosphorylated at Ser21.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser21 of human GSK-3alpha. Antibody is purified using protein G affinity chromatography.

ELISA-Peptide

ELISA-Peptide

DELIFA® data generated using biotinylated phospho- and nonphospho-peptides. Protocols and S/N values available upon request.

Description

This antibody is formulated in PBS (no BSA/no glycerol) and quality controlled for use in ELISA and other drug discovery applications. This is a sample antibody and intended for use by drug discovery scientists.

Background

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

  1. Welsh, G.I. et al. (1996) Trends Cell. Biol. 6, 274-279.
  2. Srivastava, A.K. and Pandey, S.K. (1998) Mol. Cell. Biochem. 182, 135-141.
  3. Cross, D.A. et al. (1995) Nature 378, 785-789.
  4. Nusse, R. (1997) Cell 89, 321-323.
  5. Diehl, J.A. et al. (1998) Genes Dev. 12, 3499-3511.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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