Product Pathways - Abs in PBS
Phospho-GSK-3alpha (Ser21) (27E5) Mouse mAb #5090
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| E-P | H | 51 | Mouse | IgG2b |
Applications Key:
E-P=ELISA (Peptide)
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-GSK-3alpha (Ser21) (27E5) Monoclonal Antibody can be used in high throughput kinase assays and drug discovery applications. It detects peptides derived from GSK-3alpha phosphorylated at Ser21.
Source / Purification
Monoclonal antibody is produced by immunizing mice with a synthetic phospho-peptide (KLH coupled) corresponding to residues surrounding Ser21 of human GSK-3alpha. Antibody is purified using protein G affinity chromatography.
ELISA-Peptide
DELIFA® data generated using biotinylated phospho- and nonphospho-peptides. Protocols and S/N values available upon request.
Description
This antibody is formulated in PBS (no BSA/no glycerol) and quality controlled for use in ELISA and other drug discovery applications. This is a sample antibody and intended for use by drug discovery scientists.
Background
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is an ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium, and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
- Welsh, G.I. et al. (1996) Trends Cell. Biol. 6, 274-279.
- Srivastava, A.K. and Pandey, S.K. (1998) Mol. Cell. Biochem. 182, 135-141.
- Cross, D.A. et al. (1995) Nature 378, 785-789.
- Nusse, R. (1997) Cell 89, 321-323.
- Diehl, J.A. et al. (1998) Genes Dev. 12, 3499-3511.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9331 Phospho-GSK-3α/β (Ser21/9) Antibody
- 9336 Phospho-GSK-3β (Ser9) Antibody
- 1063 GSK-3α/β (Ser21/9) Biotinylated Peptide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
