Cell Signaling Technology

Product Pathways - Apoptosis

HIPK2 Antibody #5091

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 130-140 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

HIPK2 Antibody detects endogenous levels of total HIPK2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln1045 of human HIPK2 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a mouse HIPK2 construct (+), using HIPK2 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from Raji cells, mouse brain, and PC-12 cells using HIPK2 Antibody.

Background

Members of the homeodomain-interacting protein kinase (HIPK1-4) family of serine/threonine kinases regulate gene transcription with effects on cell proliferation, differentiation, and apoptosis (1-3). HIPK1-3 are nuclear proteins that were originally described as co-repressors for homeobox transcription factors (1). HIPK proteins can interact with and/or phosphorylate many transcriptional regulators (4).

HIPK2 activated in response to DNA damage, including UV radiation and chemotherapeutic drugs, phosphorylates p53 at Ser46 to promote the transcription of pro-apoptotic p53 target genes (5-7). In addition, HIPK2 interacts with a number of transcription factors that control developmental processes, tumor suppression and apoptosis (4). The kinase is regulated by both sumoylation (8) and ubiquitination (9,10). Ubiquitination and subsequent degradation of HIPK2 is inhibited by DNA damaging agents. Caspase-dependent cleavage of HIPK2 removes the inhibitory domain and results in enhanced HIPK2 activity (11).

  1. Kim, Y.H. et al. (1998) J Biol Chem 273, 25875-9.
  2. Rochat-Steiner, V. et al. (2000) J Exp Med 192, 1165-74.
  3. Arai, S. et al. (2007) FEBS Lett 581, 5649-57.
  4. Rinaldo, C. et al. (2007) Biochem Cell Biol 85, 411-8.
  5. Hofmann, T.G. et al. (2002) Nat Cell Biol 4, 1-10.
  6. D'Orazi, G. et al. (2002) Nat Cell Biol 4, 11-9.
  7. Di Stefano, V. et al. (2004) Exp Cell Res 293, 311-20.
  8. Kim, Y.H. et al. (1999) Proc Natl Acad Sci U S A 96, 12350-5.
  9. Choi, D.W. et al. (2008) J Biol Chem 283, 4682-9.
  10. Winter, M. et al. (2008) Nat Cell Biol 10, 812-24.
  11. Gresko, E. et al. (2006) EMBO J 25, 1883-94.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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